The Centrifuge Rotor (CR) is a large life science experiment facility which will be installed in the International Space Station (ISS). It will provide artificial gravity of 2g or less by rotating up to 4 science habitats, and it will be the first such machinery to be used in space. To prevent vibration disturbance exchanges between the CR and the ISS, a soft 5 dof vibration isolation mechanism is used which cannot support the CR weight on the ground. Therefore, the CR on-orbit performance must be predicted by integrated analysis which must model all of the equipment including sensors, actuators, flexible structure, gyroscopic effects, and controllers. Here, we introduce the CR mechatronics, a verification procedure, and examples of the application of the integrated analysis which is based on the general-purpose mechanism analysis software ADAMS.