8 September 2004 Development of a highly sensitive interferometric biosensor
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Abstract
For the measurement of biomolecular interactions such as immunoreactions it is often necessary to prepare reporter molecules to detect small biomolecules. In many cases fluorescence markers are used to detect the binding between molecules. These markers, however, can influence the examined reaction. A label-free optical detection method based on the principle of a Young interferometer offers an alternative solution. This technology allows real-time, kinetic analysis of antigene-antibody reactions or the detection of a specific analyte without elaborate sample preparation. Especially reactions where it is inconvenient or impossible to use markers can be detected with this method. In this paper, an interferometric device based on a planar waveguide as sensing element is presented. The system yields a high resolution with respect to surface mass coverage and a low sensitivity towards undesired external influences. Interferometric sensors theoretically have the highest detection limits among label-free bionsensors.
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Katrin Schmitt, Katrin Schmitt, Bernd Schirmer, Bernd Schirmer, Albrecht Brandenburg, Albrecht Brandenburg, } "Development of a highly sensitive interferometric biosensor", Proc. SPIE 5461, Biophotonics New Frontier: From Genome to Proteome, (8 September 2004); doi: 10.1117/12.554231; https://doi.org/10.1117/12.554231
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