Axial localization of luminophores by partial coherence interferometry

Nicolas Sandeau; Herve Rigneault; Pierre-Francois Lenne; Hugues Giovannini

doi:10.1117/12.545661

10 September 2004 Axial localization of luminophores by partial coherence interferometry

Nicolas Sandeau, Herve Rigneault, Pierre-Francois Lenne, Hugues Giovannini

Proceedings Volume 5462, Biophotonics Micro- and Nano-Imaging; (2004) https://doi.org/10.1117/12.545661
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of a luminophore. The optical path difference between the two waves, which is related to the position of the luminophore, is recovered thanks to a second interferometer by using partial coherence interferometry demodulation technique. The proposed solution can find applications in biology for localizing with nanometric resolution a small number of tagged species.

Citation Download Citation

Nicolas Sandeau, Herve Rigneault, Pierre-Francois Lenne, and Hugues Giovannini "Axial localization of luminophores by partial coherence interferometry", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); https://doi.org/10.1117/12.545661

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available