A method for measuring cerebral blood volume of mouse using multiphoton laser scanning microscopy

Pascale Verant; Raphael Serduc; Jonathan A. Coles; Regine Farion; Chantal Remy; Boudewijn van der Sanden; Jean-Claude Vial

doi:10.1117/12.546334

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8 September 2004 A method for measuring cerebral blood volume of mouse using multiphoton laser scanning microscopy

Pascale Verant; Raphael Serduc; Jonathan A. Coles; Regine Farion; Chantal Remy; Boudewijn van der Sanden; Jean-Claude Vial

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Proceedings Volume 5463, Femtosecond Laser Applications in Biology; (2004) https://doi.org/10.1117/12.546334
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Knowledge of the volume of blood per unit volume of brain tissue is important for understanding brain function in health and disease. We describe a novel method using two-photon laser scanning microscopy to obtain the local blood volume in the cortex of the anesthetized mouse. We infused fluorescent dyes in the circulating blood and imaged the blood vessels, including the capillaries, to a depth of 400 microns below the dura at the brain surface. Blood volume was calculated by normalizing the total fluorescence measured at each depth. This method, which dispenses with form recognition, is rapid and only weakly sensitive to background noise; it could be extended to measure the leakiness of the blood vessels.

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Pascale Verant, Raphael Serduc, Jonathan A. Coles, Regine Farion, Chantal Remy, Boudewijn van der Sanden, and Jean-Claude Vial "A method for measuring cerebral blood volume of mouse using multiphoton laser scanning microscopy", Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); https://doi.org/10.1117/12.546334

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