8 September 2004 Characterization of multiphoton laser scanning device optical parameters for image restoration
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Abstract
Fluorescent nanobeads embedded in agarose and skin biopsies were used to optically characterize spatial and temporal resolution of multiphoton laser scanning devices (MPLSD). Optical sections based on two-photon excited bead fluorescence have been performed at various sample depths. Three-dimensional reconstruction of the image stacks allowed determination of the point spread function. Using calculated point spread functions to apply deconvolution procedures (e.g. Huygens software), the visualization and hence the interpretation of intradermal structures, such as extracellular matrix components in 150 μm tissue depth, was improved.
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Frank Fischer, Frank Fischer, Karsten Konig, Karsten Konig, Stefan Puschmann, Stefan Puschmann, Roger Wepf, Roger Wepf, Iris Riemann, Iris Riemann, Volker Ulrich, Volker Ulrich, Peter Fischer, Peter Fischer, } "Characterization of multiphoton laser scanning device optical parameters for image restoration", Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.545604; https://doi.org/10.1117/12.545604
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