Study of carboxyhemoglobin photodissociation with laser flash-photolysis

Vasiliy V. Kuzmin; Vladimir V. Salmin; A. B. Salmina; Alexander Sergeevich Provorov

doi:10.1117/12.578359

20 August 2004 Study of carboxyhemoglobin photodissociation with laser flash-photolysis

Vasiliy V. Kuzmin, Vladimir V. Salmin, A. B. Salmina, Alexander Sergeevich Provorov

Proceedings Volume 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V; (2004) https://doi.org/10.1117/12.578359
Event: Saratov Fall Meeting 2003, 2003, Saratov, Russian Federation

Abstract

Assessment of the carboxyhemoglobin photodissociation has been performed under the native conditions. This investigation has a great importance for the development and creation of completely new approach for the treatment of carbon monoxide poisoning based on the photoinduced dissociation of carboxyhemoglobin. Photodissociation was registered on the experimental setup with crossing laser beams were pulsed Nd:YAG laser at the second harmonics wavelength (λ=532 nm) was used as a source of photolyzing radiation. Buffered solutions of whole human peripheral blood (PBS, pH=7.4) and diluted hemolized human peripheral blood were used. We found optimal parameters for the registration of the photodissociation such as using of buffered solutions of the whole human peripheral blood with the concentration of carboxyhemoglobin around 50% detection of dissociation of carboxyhemoglobin at the maximum of absorption within the Soret's band (435 nm). Dependence of photodissociation efficiency on the concentration of the complex in the tested solutions, as well as on the photolysis radiation intensity in both types of solutions was proved.

Citation Download Citation

Vasiliy V. Kuzmin, Vladimir V. Salmin, A. B. Salmina, and Alexander Sergeevich Provorov "Study of carboxyhemoglobin photodissociation with laser flash-photolysis", Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); https://doi.org/10.1117/12.578359

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