The formation of blood sediment at low hematocrit: a kinetic study by a laser scattering technique

Bronislaw Grzegorzewski; A. Gornicki; S. Czarnecki; A. Gutsze

doi:10.1117/12.559832

4 June 2004 The formation of blood sediment at low hematocrit: a kinetic study by a laser scattering technique

Bronislaw Grzegorzewski, A. Gornicki, S. Czarnecki, A. Gutsze

Proceedings Volume 5477, Sixth International Conference on Correlation Optics; (2004) https://doi.org/10.1117/12.559832
Event: Sixth International Conference on Correlation Optics, 2003, Chernivsti, Ukraine

Abstract

In this paper the formation of erythrocyte aggregates and their sedimentation was studied by optical method. The movement and aggregation of erythrocytes produce a variation in scattered-light intensity. The well-mixed blood sample at hematocrit of 5% was vertically scanned by laser light. The scattered light intensity was continuously recorded and analyzed. The initially observed increase in the transmitted-light intensity reflected the reorganization of the structure formed by erythrocytes, and was followed by the decrease of light intensity due to an increase of blood sample optical density. In blood samples of 5% hematocrit the new, specific sedimentation curve was obtained. From this curve three different phases of sedimentation process can be distinguished. In the first phase no deposit is observed. This phase corresponds to falling of single cells as well as formation and sedimentation of small aggregates takes place. The second phase, when rapid growth of deposit is observed, corresponds to formation and sedimentation of rouleaux and large aggregates. Finally in the third phase the boundary between deposit and settling cells is slowly going down due to packing of erythrocyte aggregates. These results show that in blood samples of low hematocrit both the kinetics of erythrocyte aggregation and sedimentation process are different from those of the blood with normal and high hematocrit.

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Bronislaw Grzegorzewski, A. Gornicki, S. Czarnecki, and A. Gutsze "The formation of blood sediment at low hematocrit: a kinetic study by a laser scattering technique", Proc. SPIE 5477, Sixth International Conference on Correlation Optics, (4 June 2004); https://doi.org/10.1117/12.559832

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KEYWORDS

Blood

Plasma

Laser scattering

Light scattering

Slow light

Absorbance

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