High-sensitivity imaging method of singlet oxygen and superoxide anion in photodynamic and sonodynamic actions

Da Xing; Yonghong He; Min Hao; Qun Chen

doi:10.1117/12.572049

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29 July 2004 High-sensitivity imaging method of singlet oxygen and superoxide anion in photodynamic and sonodynamic actions

Da Xing, Yonghong He, Min Hao, Qun Chen

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Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572049
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

A novel method of photodynamic diagnosis (PDD) of cancer mediated by chemiluminescence (CL) probe is presented. The mechanism for photodynamic therapy (PDT) involves reactive oxygen species (ROS), such as singlet oxygen (¹O₂) and superoxide (O₂^-), generated by during the photochemical process. Both ¹O₂ and O₂^- can react with Cypridina luciferin analogue (FCLA), a highly selective CL probe for detecting the ROS. Chemiluminescence from the reaction of FCLA with the ROS, at about 530 nm, was detected by a highly sensitive ICCD system. The CL was markedly inhibited by the addition of 10 mmol/L sodium azide (NaN₃) in a sample solution. Similar phenomena, with lesser extents of changes, were observed at the additions of 10 μmol/L superoxide dismutase (SOD), 10 mmol/L mannitol, and 100 μg/mL catalase, respectively. This indicates that the detected CL signals were mainly from ROS generated during the photosensitization reactions. Also, the chemiluminescence method was used to detect the ROS during sonodynamic action, both in vitro and in vivo. ROS formation during sonosensitizations of HpD and ATX-70 were detected using our newly-developed imaging technique, in real time, on tumor bearing animals. This method can provide a new means in clinics for tumor diagnosis.

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Da Xing, Yonghong He, Min Hao, and Qun Chen "High-sensitivity imaging method of singlet oxygen and superoxide anion in photodynamic and sonodynamic actions", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572049

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