Use of OCT to study tumor cell growth in 3D membranes

Ying Yang; Josep Sule-Suso; Alicia J. El Haj; Paul R. Hoban; Ruikang K. Wang

doi:10.1117/12.571998

29 July 2004 Use of OCT to study tumor cell growth in 3D membranes

Ying Yang, Josep Sule-Suso, Alicia J. El Haj, Paul R. Hoban, Ruikang K. Wang

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.571998
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

Mesothelioma is a tumour, usually fatal, which arises from and invades the pleural membrane. Investigation of the tumour cell-membrane interaction will greatly increase our understanding of the invasion mechanisms, which have the potential to improve the management of the disease. In this study, a new imaging technique, optical coherence tomography (OCT), was used to monitor tumour cell invasion in artificial membranes composed of either collagen type I or Matrigel. In parallel, standard histological section analysis was performed to validate the accuracy of the monitoring by OCT. Cross-sectional images from OCT revealed that lung tumour cells invaded only when low cell seeding density (5 x 10⁵) and low collagen concentration (1.5 mg/ml) were combined. The cells could be easily differentiated from the artificial membranes and appeared as either a brighter layer on the top of the membrane or brighter spots embedded within the darker membrane. These cell-membrane morphologies matched remarkably to the standard histological section images. Our results suggest that OCT has a great potential to become a useful tool for fast and robust assessment of cell invasion.

Citation Download Citation

Ying Yang, Josep Sule-Suso, Alicia J. El Haj, Paul R. Hoban, and Ruikang K. Wang "Use of OCT to study tumor cell growth in 3D membranes", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.571998

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available