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29 July 2004 Use of OCT to study tumor cell growth in 3D membranes
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Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.571998
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom
Abstract
Mesothelioma is a tumour, usually fatal, which arises from and invades the pleural membrane. Investigation of the tumour cell-membrane interaction will greatly increase our understanding of the invasion mechanisms, which have the potential to improve the management of the disease. In this study, a new imaging technique, optical coherence tomography (OCT), was used to monitor tumour cell invasion in artificial membranes composed of either collagen type I or Matrigel. In parallel, standard histological section analysis was performed to validate the accuracy of the monitoring by OCT. Cross-sectional images from OCT revealed that lung tumour cells invaded only when low cell seeding density (5 x 105) and low collagen concentration (1.5 mg/ml) were combined. The cells could be easily differentiated from the artificial membranes and appeared as either a brighter layer on the top of the membrane or brighter spots embedded within the darker membrane. These cell-membrane morphologies matched remarkably to the standard histological section images. Our results suggest that OCT has a great potential to become a useful tool for fast and robust assessment of cell invasion.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ying Yang, Josep Sule-Suso, Alicia J. El Haj, Paul R. Hoban, and Ruikang K. Wang "Use of OCT to study tumor cell growth in 3D membranes", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.571998
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