Comparative analysis of the self-association of ethidium mono- and bis-intercalators in aqueous solution using 1H NMR spectroscopy

Maxim P. Evstigneev; Adrian A. Hernandez Santiago; Olga V. Rogova; Alexei N. Veselkov

doi:10.1117/12.569617

20 July 2004 Comparative analysis of the self-association of ethidium mono- and bis-intercalators in aqueous solution using ¹H NMR spectroscopy

Maxim P. Evstigneev, Adrian A. Hernandez Santiago, Olga V. Rogova, Alexei N. Veselkov

Author Affiliations +

Proceedings Volume 5507, XVI International Conference on Spectroscopy of Molecules and Crystals; (2004) https://doi.org/10.1117/12.569617
Event: XVI International Conference on Spectroscopy of Molecules and Crystals, 2003, Sevastopol, Ukraine

Abstract

1 D- and 2D ¹H NMR spectroscopy (500 MHz) has been used to study the self-association of a bifunctional intercalator, ethidium homodimer (EBH), in aqueous solution. A physical model describing the equilibrium ofdifferent associated forms of the homodimer in solution, including the most probable unfolded, folded conformations, dimer and trimer, has been developed. The magnitudes of equilibrium constants and thermodynamical characteristics of the complexation reactions have been obtained from analysis of the concentration and temperature dependences of proton chemical shifts of EBH molecules. Comparative analysis of the self-association parameters of ethidium homodimer and a monointercalator, ethidium bromide, enables to make some conclusions about structural features and energetic characteristics ofaggregates of aromatic molecules in solution.

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Maxim P. Evstigneev, Adrian A. Hernandez Santiago, Olga V. Rogova, and Alexei N. Veselkov "Comparative analysis of the self-association of ethidium mono- and bis-intercalators in aqueous solution using ¹H NMR spectroscopy", Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); https://doi.org/10.1117/12.569617

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