H-Bond network in biological systems: an infrared study

Joze Grdadolnik

doi:10.1117/12.570003

20 July 2004 H-Bond network in biological systems: an infrared study

Joze Grdadolnik

Proceedings Volume 5507, XVI International Conference on Spectroscopy of Molecules and Crystals; (2004) https://doi.org/10.1117/12.570003
Event: XVI International Conference on Spectroscopy of Molecules and Crystals, 2003, Sevastopol, Ukraine

Abstract

A stepwise hydration of bovine serum albumin is studied using infrared spectroscopy. For determination of the configuration interactions of hydrating H₂0 molecules to specific protein parts evaporation spectra were used. Evaporation spectra were calculated by subtraction of the spectra of hydrated protein from the spectrum of the dry protein. Evaporation spectra are further processed with a novel method, which is based on the decomposition of the original spectra into basic vectors (spectra). For detailed assignment of the basic spectra the temperature measurements and H/D exchanged were used. Upon the assignment of the basic vectors several interaction configurations of H₂0 molecules attached to specific protein sites as a function of the hydration level were proposed.

Citation Download Citation

Joze Grdadolnik "H-Bond network in biological systems: an infrared study", Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); https://doi.org/10.1117/12.570003

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