14 October 2004 Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence
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Abstract
We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to self-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the 6x-histidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.
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Peng Zhang, Peng Zhang, Shi-You Ding, Shi-You Ding, Qi Xu, Qi Xu, Steve Smith, Steve Smith, Garry Rumbles, Garry Rumbles, Michael E. Himmel, Michael E. Himmel, } "Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence", Proc. SPIE 5513, Physical Chemistry of Interfaces and Nanomaterials III, (14 October 2004); doi: 10.1117/12.560231; https://doi.org/10.1117/12.560231
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