A double trap for single-molecule manipulation at opaque and microstructured surfaces

Olivier Legrand; Denis Cote; Ulrich Bockelmann

doi:10.1117/12.559795

18 October 2004 A double trap for single-molecule manipulation at opaque and microstructured surfaces

Olivier Legrand, Denis Cote, Ulrich Bockelmann

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.559795
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

We describe a double optical trap for micromanipulation of biomolecules. This set-up allows to bring a DNA molecule grafted between two beads in contact with microelectrodes, in order to perform electrical measurements at the single molecule level in a well-controlled geometry. The use of an opaque surface like silicon compatible with lithography imposes to work in a reflection configuration with the same objective for trapping and imaging the bead. The experimental constraints raised by this optical configuration are detailed. By combining drag force and optical trapping, we are able to trap separately the two beads and to perform force versus extension measurements in this reflection configuration. Finally, deposition of one stretched DNA molecule on Au electrodes fabricated by electron beam lithography on silicon wafers is shown.

Citation Download Citation

Olivier Legrand, Denis Cote, and Ulrich Bockelmann "A double trap for single-molecule manipulation at opaque and microstructured surfaces", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.559795

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