CONFERENCE PROCEEDINGS
Advanced Search >
Home > Proceedings > Volume 5514 > Article
Translator Disclaimer
18 October 2004 Advantages and disadvantages in using oil immersed microscope objectives for optical trapping
Radu Malureanu; Enrico Ferrari; Enzo Di Fabrizio
Author Affiliations +
Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.559402
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States
Abstract
Since the first demonstrations of optical trapping, both theoretical and experimental parts of this technique evolved. With all this, the main problem when trapping in the Mie regime is due to the limited numerical aperture a microscope objective has. In literature one finds characterizations of “classical” microscope lens or, at most, water immersed ones. In this paper we are analysing the forces generated in an optical tweezers setup using oil immersed microscope objective and having as entrapped particles water-immersed silica beads. Using such a set-up, we can take advantage of the numerical aperture an oil-immersed objective can reach. This numerical aperture can have a value as high as 1.4. From Roosen's 1 and Ashkin's 2 formulas, we calculated the forces involved in our experiment. We observed that the entrapping range on the optical path axis is larger and asymmetric. This generates the possibility to build optical catapult and optical tweezers in the same time, changing only the distance from the sample to the entrapment point. One of the disadvantages of optical trapping in these conditions is that the focus point and the entrapment one can be different. This fact generates the need of using a second microscope for inspecting the entrapped particle so the optical setup is more complicated. To our belief, this set-up for optical tweezers can have big advantages in the field of optical trapping mainly due to the not so strict trapping spatial conditions.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Radu Malureanu, Enrico Ferrari, and Enzo Di Fabrizio "Advantages and disadvantages in using oil immersed microscope objectives for optical trapping", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.559402
ACCESS THE FULL ARTICLE
PERSONAL SIGN IN
Full access may be available with your subscription
 
Forgot your username?
Forgot your password?
No SPIE account? Create an account
Institutional Access:
Sign in with your institutional credentials
PURCHASE THIS CONTENT
INTERESTED IN A FREE CORPORATE TRIAL?
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $14.40
Non-members: $18.00 ADD TO CART
PROCEEDINGS
 5 PAGES
DOWNLOAD PDF SAVE TO MY LIBRARY
SHARE
GET CITATION
< Previous Article
|
Next Article >
Advertisement
Advertisement
KEYWORDS
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top