18 October 2004 Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study
Author Affiliations +
Abstract
We have investigated the effect of ionic strength on electrically evoked membrane tether force using optical tweezers. Membrane tethers from cochlear outer hair cells (OHCs) and human embryonic kidney (HEK) cells exhibited mechanical response to applied voltage stimuli over a wide frequency range. The electrically evoked variations in the tether force were probed by an optically-trapped microsphere, the image of which was projected on a quadrant photodiode for dynamic measurement of its displacements. Compared to normal saline (140mM NaCl), low ionic-strength solution (10mM NaCl) blocked the electrically evoked tether force for both OHCs and HEK cells. As the Debye length for membrane bilayer was estimated to increase from approximately 0.75nm to 1.88nm, the internal repulsive pressure of the membrane tethers rose consequently, resulting in the enlargement in the equilibrium tether diameter and the decrease in the tether force.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Feng Qian, Feng Qian, William E. Brownell, William E. Brownell, Bahman Anvari, Bahman Anvari, } "Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.560021; https://doi.org/10.1117/12.560021
PROCEEDINGS
6 PAGES


SHARE
Back to Top