Optical probing of specific and nonspecific interactions with nanometer resolution

Jakob Kisbye Dreyer; Poul Martin Hansen; Lene Broeng Oddershede

doi:10.1117/12.559622

18 October 2004 Optical probing of specific and nonspecific interactions with nanometer resolution

Jakob Kisbye Dreyer, Poul Martin Hansen, Lene Broeng Oddershede

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.559622
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Using optical tweezers combined with video based methods we probe non-specific interactions between polystyrene beads and surfaces at different electrolyte concentrations. We present force-distance relationships and using statiscally based maximum likelihood methods, which significantly improves spatial resolutions, the interaction potentials are also found. At low electrolyte concentrations the interaction potentials are in agreement with DLVO theory, but at high electrolyte concentrations the interaction potentials deviate both from DVLE and Lifshitz theories. Also, we probe the specific interaction between biotin and streptavidin. However, these measurements are complicated because the interaction is of very short range and a streptavidin coated polystyrene bead adheres to a biotin coated surface as soon as it encounters the surface during its Brownian motion in the trap.

Citation Download Citation

Jakob Kisbye Dreyer, Poul Martin Hansen, and Lene Broeng Oddershede "Optical probing of specific and nonspecific interactions with nanometer resolution", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.559622

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