Spherical aberration correction for optical tweezers

Jochen Arlt; Laurence Wilson; Eirini Theofanidou; William J. Hossack

doi:10.1117/12.555824

18 October 2004 Spherical aberration correction for optical tweezers

Jochen Arlt, Laurence Wilson, Eirini Theofanidou, William J. Hossack

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.555824
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Typical optical tweezers setups use high numerical aperture oil-immersion objectives to trap particles suspended in an aqueous medium. When trapping deep inside a sample or out of the imaging plane the quality of the trap in such a system deteriorates due to optical aberrations caused by the refractive index mismatch at the glass-water interface. We investigate this effect experimentally by monitoring the two-photon fluorescence of trapped dye-stained polystyrene spheres. We consider the effect of the numerical aperture on the trap quality and also partly corrected the aberrations by optimising the fluorescence signal using an adaptive deformable membrane mirror.

Citation Download Citation

Jochen Arlt, Laurence Wilson, Eirini Theofanidou, and William J. Hossack "Spherical aberration correction for optical tweezers", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.555824

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available