Manipulation of DNA molecules using optical techniques for optically assisted DNA computing

Yusuke Ogura; Fumika Sumiyama; Takashi Kawakami; Jun Tanida

doi:10.1117/12.559241

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8 October 2004 Manipulation of DNA molecules using optical techniques for optically assisted DNA computing

Yusuke Ogura, Fumika Sumiyama, Takashi Kawakami, Jun Tanida

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Proceedings Volume 5515, Nanoengineering: Fabrication, Properties, Optics, and Devices; (2004) https://doi.org/10.1117/12.559241
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

DNA computing is an interesting computational paradigm utilizing reactive nature of DNA. The DNA computing realizes massively parallel computation because a large number of DNAs are processed in parallel. However, the computational functionality is restricted by the number of DNA molecules and DNA reactions that can be employed. As a new computational scheme, we are studying optically assisted DNA computing, which utilizes flexibility in generating light fields and parallelism of DNA reactions. Toward the goal, we experimentally verified methods for translating DNA molecules and controlling DNA reactions locally by using optical techniques. An optical manipulation method with VCSEL array sources is applied to translate DNA. We succeeded to translate two DNA clusters, which consisted of many DNA molecules attached to particles, in different directions simultaneously by switching the emission pattern of the VCSEL array. The reaction of DNA is controlled by irradiating with a laser beam. Experimental results demonstrated that double stranded DNAs, which were immobilized to the surface of a particle or a substrate, were denaturated at a resolution of several micrometer. The methods make possible to deal with a set of DNAs selectively and are useful in executing flexible operations for computing.

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Yusuke Ogura, Fumika Sumiyama, Takashi Kawakami, and Jun Tanida "Manipulation of DNA molecules using optical techniques for optically assisted DNA computing", Proc. SPIE 5515, Nanoengineering: Fabrication, Properties, Optics, and Devices, (8 October 2004); https://doi.org/10.1117/12.559241

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