A mobile-intensified-femtosecond-fiber-laser-based TPF spectrometer for early diagnosis of malignant melanoma

Klaus Teuchner; Stephan Mory; Dieter Leupold

doi:10.1117/12.557182

15 October 2004 A mobile-intensified-femtosecond-fiber-laser-based TPF spectrometer for early diagnosis of malignant melanoma

Klaus Teuchner, Stephan Mory, Dieter Leupold

Author Affiliations +

Proceedings Volume 5516, Nonlinear Optical Transmission and Multiphoton Processes in Organics II; (2004) https://doi.org/10.1117/12.557182
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

The fluorescence of melanin in human skin tissue reflects the structural changes in the process of skin cancer progression towards malignant melanoma. A selectively excitation of melanin in skin tissue, however, is only possible by stepwise two-photon absorption (TPA) in the NIR spectral range (λ_exc ≈ 800 nm). Due to the very short lifetime of the energy level populated by the absorption of the first photon (< 5 ps) an effective TPA process can be only achieved with laser pulses on the femtosecond time scale. To use the two-photon excited fluorescence (TPF) of melanin for early diagnosis of malignant melanoma a small, mobile TPF spectrometer was developed. It consists (i) of a fiber laser the radiation of which was amplified in a short pulse nitrogen laser pumped dye cell, (ii) an articulated arm with special mirrors for nearly lossless direction of the fs laser beam to the skin position of investigation, (iii) a camera objective which include visualization and excitation as well as acquisition of fluorescence and (iv) a monochromator with a multichannel photomultiplier and a PC with special software for spectrometer control and data processing. First investigations of the equipment concerning the early diagnosis of malignant melanoma were carried out.

Citation Download Citation

Klaus Teuchner, Stephan Mory, and Dieter Leupold "A mobile-intensified-femtosecond-fiber-laser-based TPF spectrometer for early diagnosis of malignant melanoma", Proc. SPIE 5516, Nonlinear Optical Transmission and Multiphoton Processes in Organics II, (15 October 2004); https://doi.org/10.1117/12.557182

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available