Three-dimensional imaging with high spatial resolution: scanning microscopy

Manuel Martinez-Corral; Genaro Saavedra

doi:10.1117/12.557871

4 November 2004 Three-dimensional imaging with high spatial resolution: scanning microscopy

Manuel Martinez-Corral, Genaro Saavedra

Proceedings Volume 5556, Photonic Devices and Algorithms for Computing VI; (2004) https://doi.org/10.1117/12.557871
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Two-photon scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of diffractive elements that properly shape the 3D point spread function. Our diffractive elements have been designed to work in the nonparaxial regime and are immune to a harmful effect, usually inherent to high-angle focusing systems, named as depolarization

Citation Download Citation

Manuel Martinez-Corral and Genaro Saavedra "Three-dimensional imaging with high spatial resolution: scanning microscopy", Proc. SPIE 5556, Photonic Devices and Algorithms for Computing VI, (4 November 2004); https://doi.org/10.1117/12.557871

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