4 November 2004 Three-dimensional imaging with high spatial resolution: scanning microscopy
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Abstract
Two-photon scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of diffractive elements that properly shape the 3D point spread function. Our diffractive elements have been designed to work in the nonparaxial regime and are immune to a harmful effect, usually inherent to high-angle focusing systems, named as depolarization
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Manuel Martinez-Corral, Genaro Saavedra, "Three-dimensional imaging with high spatial resolution: scanning microscopy", Proc. SPIE 5556, Photonic Devices and Algorithms for Computing VI, (4 November 2004); doi: 10.1117/12.557871; https://doi.org/10.1117/12.557871
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KEYWORDS
Point spread functions

Microscopes

Luminescence

3D image processing

Microscopy

Imaging systems

Photons

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