22 October 2004 Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections
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Abstract
An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined using h-dome extraction and image filtering, based on grayscale reconstruction. Erroneous locations are eliminated based on lattice regularity. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and classified using a Gaussian mixture model. Application to a number of micrographs and comparison with manual classifications of orientations shows that the system is effective in many cases.
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Bjarni Bodvarsson, Bjarni Bodvarsson, Soren Klim, Soren Klim, Stig Mortensen, Stig Mortensen, Martin Morkebjerg, Martin Morkebjerg, James Chen, James Chen, Julian R. Maclaren, Julian R. Maclaren, Chun Hong Yoon, Chun Hong Yoon, Pradeep K. Luther, Pradeep K. Luther, John M. Squire, John M. Squire, Andrew Bainbridge-Smith, Andrew Bainbridge-Smith, Philip J. Bones, Philip J. Bones, Rick P. Millane, Rick P. Millane, } "Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections", Proc. SPIE 5562, Image Reconstruction from Incomplete Data III, (22 October 2004); doi: 10.1117/12.562357; https://doi.org/10.1117/12.562357
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