Application of methods of optimal design of experiments to study optical properties of blood

E. K. Naumenko

doi:10.1117/12.577093

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26 August 2004 Application of methods of optimal design of experiments to study optical properties of blood

E. K. Naumenko

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Proceedings Volume 5566, Optical Security and Safety; (2004) https://doi.org/10.1117/12.577093
Event: Optical Security and Safety, 2003, Warsaw, Poland

Abstract

An optical model of erythrocyte aggregation with blood-plasma capture is proposed. The shape of erythrocytes was approximated by a circular cylinder with a concave ball-like base. The ratios relating the optical constants averaged over the volume of the aggregates to the geometric parameters of erythrocytes and the number of the aggregated erythrocytes have been obtained. In the homogeneous sphere approximation we have calculated the following basic characteristics of scattering, determining the radiation transfer in a scattering medium under the multiple scattering conditions: extinction coefficient, absorption coefficient, photon survival probability, first coefficient in the expansion of the scattering indicatrix in terms of the Legandre polynomials. Three variants of an elementary volume of blood were examined: a suspension of isolated individual erythrocytes, a suspension containing aggregates formed without blood-plasma capture, and a suspension in which erythrocyte aggregation occured with capture of blood plasma by the concave bases of erythrocytes. The methods of designing an optical experiment were used for analyzing the calculation results. It is shown that the processes of erythrocyte aggregation and possible blood-plasma capture, occurring as a result of the formation of aggregates, can cause significant changes in the optical characteristics of an elementary volume of blood. Simple mathematical expressions relating the value of the diffuse-reflection factor of radiation reflected from a blood layer to the parameters and the number of erythrocytes in the composition of the aggregates have been obtained. The statistical significance of the influence of the optical and geometric parameters of erythrocytes has been estimated.

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E. K. Naumenko "Application of methods of optimal design of experiments to study optical properties of blood", Proc. SPIE 5566, Optical Security and Safety, (26 August 2004); https://doi.org/10.1117/12.577093

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