16 November 2004 Properties and potential of a polarization technique for the separation of the overlapping fluorescence and elastic scattering applied to algae in seawater
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Abstract
We present the results of recent experiments and analysis on the polarization discrimination technique developed by us to separate elastic reflectance and fluorescence components resulting from white light illumination of algae in seawater. The technique uses the polarized properties of elastically scattered light and unpolarized properties of fluorescence to separate the two. The approach was successfully applied to measurements on four types of algae of different sizes and shapes in the laboratory, with both polarized and unpolarized illumination sources. The procedure is shown to be effective for extraction of the chlorophyll fluorescence in the 685 nm region from reflectance of algae dominated by chlorophyll pigments with different angles of illumination for both polarized and unpolarized light sources, including sunlight. The results are compared with other methods for the estimation of chlorophyll concentrations, such as blue/green ratio, and the fluorescence line height above the baseline. We also report the results of experiments which examine the impact of surface roughness on the efficacy of the technique for measurements above the surface.
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Alexander A. Gilerson, Alexander A. Gilerson, Jing Zhou, Jing Zhou, Adrian Gill, Adrian Gill, Barry M. Gross, Barry M. Gross, Fred Moshary, Fred Moshary, Samir Ahmed, Samir Ahmed, } "Properties and potential of a polarization technique for the separation of the overlapping fluorescence and elastic scattering applied to algae in seawater", Proc. SPIE 5569, Remote Sensing of the Ocean and Sea Ice 2004, (16 November 2004); doi: 10.1117/12.565296; https://doi.org/10.1117/12.565296
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