FRET-based luminescence sensors for carbohydrates and glycoproteins analysis

Zeev Rosenzweig; Nitsa Rosenzweig; Gabriela Blagoi

doi:10.1117/12.570450

7 December 2004 FRET-based luminescence sensors for carbohydrates and glycoproteins analysis

Zeev Rosenzweig, Nitsa Rosenzweig, Gabriela Blagoi

Proceedings Volume 5588, Smart Medical and Biomedical Sensor Technology II; (2004) https://doi.org/10.1117/12.570450
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

This paper describes the development of novel particle-based fluorescence resonance energy transfer (FRET) biosensors. It describes the fundamentals of FRET in heterogeneous systems and the application of the new sensors in monitoring the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins. The sensing approach is based on FRET between fluorescein (donor) labeled lectin molecules, adsorbed on the surface of micrometric polymeric beads, and polymeric dextran molecules labeled with Texas Red (acceptor). The FRET signal of the sensor decreases in the presence of carbohydrates or glycoproteins that inhibit the binding of Texas Red-labeled dextran molecules to the lectinic binding sites. The new FRET sensors could discriminate between carbohydrates and glycoproteins based on their binding affinity to the FRET sensing particles. Thery were also used for quantitative analysis of carbohydrates and glycoproteins in aqueous samples.

Citation Download Citation

Zeev Rosenzweig, Nitsa Rosenzweig, and Gabriela Blagoi "FRET-based luminescence sensors for carbohydrates and glycoproteins analysis", Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); https://doi.org/10.1117/12.570450

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