8 December 2004 Time domain transmission line dielectric probe for detection of biomolecular interactions
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Proceedings Volume 5591, Lab-on-a-Chip: Platforms, Devices, and Applications; (2004) https://doi.org/10.1117/12.580138
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States
Abstract
A probe was developed to detect biomolecular binding events on a substrate in the microwave regime in real time and without labels. The probe consists of a coplanar transmission line fabricated on a glass slide that can detect dielectric changes in close proximity of the substrate-liquid interface. The probe behavior was evaluated by adsorbing polyelectrolyte monolayers of alternating charge. Biomolecular detection was demonstrated by immobilizing protein A on the glass surface and detecting rabbit IgG molecules in a flow channel. The sensitivity of the probe was conservatively estimated to be ~100 pg/mm2.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Qin Chen, Qin Chen, Daniel B. Roitman, Daniel B. Roitman, Andre Knoesen, Andre Knoesen, } "Time domain transmission line dielectric probe for detection of biomolecular interactions", Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); doi: 10.1117/12.580138; https://doi.org/10.1117/12.580138
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