8 December 2004 Time domain transmission line dielectric probe for detection of biomolecular interactions
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Proceedings Volume 5591, Lab-on-a-Chip: Platforms, Devices, and Applications; (2004) https://doi.org/10.1117/12.580138
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States
Abstract
A probe was developed to detect biomolecular binding events on a substrate in the microwave regime in real time and without labels. The probe consists of a coplanar transmission line fabricated on a glass slide that can detect dielectric changes in close proximity of the substrate-liquid interface. The probe behavior was evaluated by adsorbing polyelectrolyte monolayers of alternating charge. Biomolecular detection was demonstrated by immobilizing protein A on the glass surface and detecting rabbit IgG molecules in a flow channel. The sensitivity of the probe was conservatively estimated to be ~100 pg/mm2.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Qin Chen, Qin Chen, Daniel B. Roitman, Daniel B. Roitman, Andre Knoesen, Andre Knoesen, "Time domain transmission line dielectric probe for detection of biomolecular interactions", Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); doi: 10.1117/12.580138; https://doi.org/10.1117/12.580138
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