Improvement of hybridization signals of colorimetric gene detection based on porous polypropylene membranes

Libo Nie; Ancun Zhou; Hong Chen; Quanguo He; Nongyue He

doi:10.1117/12.573537

29 December 2004 Improvement of hybridization signals of colorimetric gene detection based on porous polypropylene membranes

Libo Nie, Ancun Zhou, Hong Chen, Quanguo He, Nongyue He

Author Affiliations +

Proceedings Volume 5593, Nanosensing: Materials and Devices; (2004) https://doi.org/10.1117/12.573537
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Porous polypropylene (PP) membranes were modified by the plasma treatment in order to graft amino functional group (-NH₂) onto the membrane surface. Oligonucleotides were in situ synthesized on the aminated polypropylene support. Gold nanoparticle labeled DNAs were bybridized to the synthesized oligonucleotide array. The membranes were exposed to the Silver Enhance Solution for singla amplification. The Hybridization signals of amino plasma-grafted polypropylene membranes were stronger than the commercial polyacrylamide modified polyproplylene membranes that load 0.07 μmol/cm² free primary amino functions. Complementary and mismatched sequences were clearly distinguished. The diameter of nanogold particles and the concentration of thiol DNA modified gold nanoparticles were investigated to improve the hybridization signals. Bigger nanoparticle diameter, as well as higher concentration of thiol DNA modified gold nanoparticles lead to stronger hybridization signals.

Citation Download Citation

Libo Nie, Ancun Zhou, Hong Chen, Quanguo He, and Nongyue He "Improvement of hybridization signals of colorimetric gene detection based on porous polypropylene membranes", Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); https://doi.org/10.1117/12.573537

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