25 October 2004 Proposal of spectroscopy-tomography of single cell
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Currently, diagnosis of cancer is performed by biopsy, whereby medical doctors observe a removed specimen, focusing their attention on morphological changes in the cell sequence and cell nuclei. For early cancer, the only effect is a slight increase in the size of the cell nuclei in comparison with normal cells. Based on medical knowledge, it is presumed that an extremely small amount of a specific protein may be contained in a cell nucleus. We propose spectroscopy-tomography of single cell to measure slight changes in this protein. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single cell rotating method. We propose variable phase-contrast spectrometry as the high spatial resolution spectrometry and proximal two-beam optical tweezers as the precise rotating method. By these methods, we can obtain a 3-dimensional distribution of the cell components to a high spatial resolution. We verified the accuracy of variable phase-contrast spectrometry by measuring the height of a diffraction grating. We confirmed that a microsphere can be rotated by proximal two-beam optical tweezers.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Toshiki Yasokawa, Toshiki Yasokawa, Ichirou Ishimaru, Ichirou Ishimaru, Fumikazu Oohira, Fumikazu Oohira, Ryoji Hyodo, Ryoji Hyodo, Hiroaki Kobayashi, Hiroaki Kobayashi, Akihiro Hayashi, Akihiro Hayashi, Y. Inoue, Y. Inoue, Katsumi Ishizaki, Katsumi Ishizaki, "Proposal of spectroscopy-tomography of single cell", Proc. SPIE 5604, Optomechatronic Micro/Nano Components, Devices, and Systems, (25 October 2004); doi: 10.1117/12.571134; https://doi.org/10.1117/12.571134

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