10 September 2004 Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment
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Proceedings Volume 5610, Laser Florence 2003: A Window on the Laser Medicine World; (2004) https://doi.org/10.1117/12.584388
Event: Laser Florence 2003: A Window on the Laser Medicine World, 2003, Florence, Italy
Abstract
Hydroxypyrimidine 5-fluorouracil (5-FU) belongs to the cytostatics group known as antimetabolites. The effect of UV irradiation on 5-FU was investigated by absorption and fluorescence spectroscopy. The study of the photosensitizer properties of 5-FU was made since their effects could be enhanced by exposure to UV radiation at different doses. Solutions 2.5x10-4M in natural saline water (0.8% NaCl), irradiated with optical beams emitted by N2 laser and UV Hg classic lamp, were used. The 5-FU was chosen due to its strong absorption along a large spectral range which makes possible the fluorescence excitation in UV. The absorption spectra exhibit bands between 250 - 450 nm. The emission fluorescence was measured in the 400-550 nm spectral range, with λex=320 and 350 nm for samples irradiated with Hg lamp and with λex=360 nm for samples irradiated with N2 laser. The excitation fluorescence was measured in the spectral range 200-400 nm, with λem=440 nm for samples irradiated with N2 laser. The spectra reveal a fluorescence enhancement with the exposure time, with a maximum at 3 min due to the transformation of 5-FU molecule into a fluorescent tautomeric form. The destruction more rapid than usual of the neovascularisation was observed for conjunctive of rabbit eyes, when they are impregnated with 5-FU solution and exposed to incoherent UV and visible light.
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Mihail-Lucian Pascu, Mihail-Lucian Pascu, Benone D. Carstocea, Benone D. Carstocea, Mihail Brezeanu, Mihail Brezeanu, Letitia Voicu, Letitia Voicu, Angela Staicu, Angela Staicu, Doina M. Gazdaru, Doina M. Gazdaru, Ruxandra A. Pascu, Ruxandra A. Pascu, } "Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment", Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); doi: 10.1117/12.584388; https://doi.org/10.1117/12.584388
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