29 December 2004 A novel UV-laser source for fluorescence excitation of proteins
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Abstract
A novel, compact and robust UV laser has been developed for laser induced fluorescence spectroscopy of biomolecules in the spectral region from 290 nm to 345 nm. It was based on a frequency-doubled passively Q-switched Nd:YAG laser, emitting at 532 nm, which was pumping a periodically poled KTiOPO4 optical parametric oscillator with intra-cavity sum-frequency mixing in a BBO crystal. The output was generated in two branches in the UV, 293 nm and 343 nm, with pulse widths of 1.8 ns and pulse repetition rate of 100 Hz. These wavelengths were then used for fluorescence experiments of bioagents.
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Mikael Tiihonen, Mikael Tiihonen, Valdas Pasiskevicius, Valdas Pasiskevicius, Fredrik Laurell, Fredrik Laurell, Mikael Lindgren, Mikael Lindgren, "A novel UV-laser source for fluorescence excitation of proteins", Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.568436; https://doi.org/10.1117/12.568436
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