29 December 2004 Multispectral-diode-laser-induced fluorescence biological particle sensor
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Abstract
Laser-induced fluorescence (LIF) provides a real-time technique for detecting micron-size airborne pathogens. Early LIF biological particle sensors used harmonic generation of UV in solid-state lasers to excite fluorescence. UV diode lasers have several key advantages over traditional lasers: a greater selection of wavelengths for the efficient and selective excitation of specific fluorescent biological compounds; continuous output so that all sampled particles are interrogated; and the ability to combine several UV diode lasers emitting at different wavelengths into a compact multiple-wavelength source for simultaneously exciting several biofluorophores. The coincident detection of multiple biofluorophores is expected to markedly improve discrimination of airborne pathogens from non-biological background aerosols. In this paper, we describe BioLert 2x16C5+1 - a LIF bio-particle sensor with two diode lasers, detection of sixteen fluorescence emission bands bundled into five user-defined linear combinations, and an elastic scatter detector. BioLert 2x16C5+1 also features fluorescence photon counting for sensitivity sufficient to distinguish between single bacterial spores and similar size inert particles, improved signal processing for optimally distinguishing between airborne pathogens and harmless particles, and a highly integrated air sampling system.
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Geoffrey A. Wilson, Geoffrey A. Wilson, Richard K. DeFreez, Richard K. DeFreez, } "Multispectral-diode-laser-induced fluorescence biological particle sensor", Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.578854; https://doi.org/10.1117/12.578854
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