Optical classification of bioaerosols using UV fluorescence and IR absorption spectroscopy

Alan L. Huston; Vasanthi Sivaprakasam; Cathy S. Scotto; Horn-Bond Lin; Jay D. Eversole; Abraham Schultz; Jeff M. Willey

doi:10.1117/12.578062

29 December 2004 Optical classification of bioaerosols using UV fluorescence and IR absorption spectroscopy

Alan L. Huston, Vasanthi Sivaprakasam, Cathy S. Scotto, Horn-Bond Lin, Jay D. Eversole, Abraham Schultz, Jeff M. Willey

Proceedings Volume 5617, Optically Based Biological and Chemical Sensing for Defence; (2004) https://doi.org/10.1117/12.578062
Event: European Symposium on Optics and Photonics for Defence and Security, 2004, London, United Kingdom

Abstract

A partnership that includes the Naval Research Laboratory (NRL), MIT Lincoln Laboratories and the Edgewood Chemical and Biological Command is engaged in an effort to develop optical techniques for the rapid detection and classification of biological aerosols. This paper will describe two efforts at NRL: development of an improved UV fluorescence front-end trigger and the use of infrared absorption spectroscopy to classify biological aerosol particles. UV Laser-induced fluorescence (UVLIF) has been demonstrated to provide very high sensitivity for differentiating between biological and inorganic aerosol particles. Unfortunately, current UVLIF systems have unacceptably high false alarm rates due to interferences from man made and naturally occurring organic and biological particulates. We have developed a two-wavelength, UVLIF technique that offers a higher level of discrimination than is possible using single wavelength UVLIF. Infrared absorption spectroscopy coupled with multivariate analysis demonstrates a high potential for differentiation among members of biological and chemical sample classes. Two-wavelength UVLIF in combination with the IR interrogation of collected bioaerosols could provide a rapid, reagentless approach to specific classification of biological particles according to an operational level of discrimination - the degree of particle characterization required in order to signal the presence of pathogenic material.

Citation Download Citation

Alan L. Huston, Vasanthi Sivaprakasam, Cathy S. Scotto, Horn-Bond Lin, Jay D. Eversole, Abraham Schultz, and Jeff M. Willey "Optical classification of bioaerosols using UV fluorescence and IR absorption spectroscopy", Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); https://doi.org/10.1117/12.578062

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