Application of the FRET method for monitoring the dynamics of caspase-3 activation during apoptosis in living cells

Tongsheng Chen; Da Xing

doi:10.1117/12.572753

18 January 2005 Application of the FRET method for monitoring the dynamics of caspase-3 activation during apoptosis in living cells

Tongsheng Chen, Da Xing

Author Affiliations +

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.572753
Event: Photonics Asia, 2004, Beijing, China

Abstract

Activation of caspase-3 is a central event in apoptosis. A fluorescence techniques, fluorescence resonance energy transfer (FRET), was used to study the dynamic of caspase-3 activation during apoptosis induced by tumor necrosis factor TNF-α in living cells. The FRET probe consists a CFP (cyan fluorescent protein) and a Venus (YFP mutant, yellow fluorescent protein) with a specialized linker containing the caspase-3 cleavage sequence: DEVD (Luo et al., 2001). Human lung adenocarcinoma cell line (ASTC-a-1) were stably expressed with the FRET probe and then were treated by TNF-α, respectively. Experimental results showed that FRET could monitor more insensitively the dynamic of caspase-3 activation in real-time in vivo, and this technique will be highly useful for correlating the caspase-3 activation with other apoptotic events and for rapid-screening of potential drugs that may target the apoptotic process.

Citation Download Citation

Tongsheng Chen, Tongsheng Chen, Da Xing, Da Xing, "Application of the FRET method for monitoring the dynamics of caspase-3 activation during apoptosis in living cells", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.572753; https://doi.org/10.1117/12.572753

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