18 January 2005 Novel confocal microarray scanner for fluorescence detection
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Abstract
A novel confocal microarray scanner was introduced, which employed a 532nm laser and a 635nm laser to excite Cy3 and Cy5 fluorophores respectively. The fluorescent signal was detected using a photomultiplier tube (PMT) sequentially. One dimension scanning of the microarray slide was performed by a telecentric objective with a moving coil optical scanner; the other dimension was scanned by a stepping motor driving the precise guidance. Experimental results show that scanning resolution of the presented microarray scanner can reach 5 microns, its dynamic range is near 4 orders of magnitude and the limit of detection is 1.12 about fluors per square micron. The cross-talk error is eliminated almost completely by its sequential scanning mode.
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Liqiang Wang, Liqiang Wang, Xuxiang Ni, Xuxiang Ni, Zukang Lu, Zukang Lu, } "Novel confocal microarray scanner for fluorescence detection", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.573428; https://doi.org/10.1117/12.573428
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