18 January 2005 Novel design for suspension array detection system
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This paper presents a novel method for establishing a two-dimensional laminar fluidic suspension array which is analyzed by using time delay integration (TDI) CCD imaging technology in parallel. The method will make suspension array technology (SAT) bear high throughput as well as its flexibility. Basically, bioassays are conducted on the surface of fluorescent-dyed beads. With each bead set (i.e., multiple beads with the same fluorescent signature) having a slightly different fluorescent signature, probes are first attached to a particular bead set and then hybridized with labeled samples or targets. Two different kinds of encoding dyes are excited by red laser (635 nm, 20mw), their emission wave length are 660nm, 720nm, respectively. Fluorescent dye of reporter molecules was excited by green laser (532nm, 20mw), emitted at 580 nm. The liquid sample was pumped into micro-reservoir by a linear motor. As the velocity of liquid sample is so slow (10mm/s) it is easy to form a laminar fluidic field in the middle of the micro-reservoir. In the direction of laser propagation the size of reservoir is 0.1mm so the laminar liquid can be treated as a two-dimensional fluidic plane. The size of detection area depends on size of micro-sphere and CCD imaging area. The three kinds of fluorescence signals were focused by a lens and then split by mirrors. Fluorescence pass through three band-pass filters (±20nm) before collected by three TDI-CCDs respectively. With these high-quality filters the cross-talk between signals was diminished significantly. The analysis speed is about 2x103 micro-spheres per second, which is much higher than that obtained from currently cytometry method (about 102 micro-spheres to the same size micro-spheres).
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Guoxiong Xu, Guoxiong Xu, Yan Shi, Yan Shi, Xuxiang Ni, Xuxiang Ni, Zukang Lu, Zukang Lu, "Novel design for suspension array detection system", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.575022; https://doi.org/10.1117/12.575022

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