Optical trapping and Raman spectroscopy of single living cells: principle and applications

Jianliao Deng; Qing Wei; Yuzhu Wang; Yong Qing Li

doi:10.1117/12.570058

18 January 2005 Optical trapping and Raman spectroscopy of single living cells: principle and applications

Jianliao Deng, Qing Wei, Yuzhu Wang, Yong Qing Li

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.570058
Event: Photonics Asia, 2004, Beijing, China

Abstract

This paper reports the principle and applications of the combination technique of optical trapping and Raman spectroscopy for real-time analysis of single living cells. We demonstrate that the information of each substance inside a captured cell can be retrieved by the Raman spectrum of the cell. The effect of alcohol solution on single human Red Blood Cell (RBC) is investigated using near-infrared laser tweezers Raman spectroscopy (LTRS). The significant difference between the spectrum of fresh RBC and the spectrum of RBC exposed to alcohol is observed due to the degradation of RBC. We also present the preliminary study result on the diagnosis of colorectal cancer using LTRS system.

Citation Download Citation

Jianliao Deng, Qing Wei, Yuzhu Wang, and Yong Qing Li "Optical trapping and Raman spectroscopy of single living cells: principle and applications", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.570058

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