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18 January 2005 Sonodynamic diagnosis of cancer with sonosensitization of ATX-70 mediated by chemiluminescence probe
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In order to develop a novel diagnostic technique, as an adjunct modality to SDT, that uses the gallium-porphyrin derivative ATX-70 as a sonosensitiser mediated by a chemiluminescent probe to produce sonochemiluminescence (SCL) for imaging and localization of cancer within tissue in vivo. The fluoresceinyl Cyprodina luminescent analogue (FCLA) was used as an optical reporter for singlet oxygen generated from the sonosensitisation reaction of ATX-70 in the model solutions and in the in vivo nude mice bearing mammary cancer. The sonosensitized chemiluminescence from the reaction of FCLA with 1O2 was detected by a highly sensitive light-detector. The effect of FCLA with different doses on cell viability was also assessed with MTT assay. The results showed with the FCLA as the reporter, there is clear evidence that the aqueous solution of ATX-70 exposed to ultrasound forms 1O2 in the system. In the in vivo experiments, the tumor-bearing mice are imaged with a great contrast during sonosensitization of ATX-70 mixed with FCLA as the SCL probe. No significant cytotoxity was detected at the different FCLA doses. The results indicated that a novel cancer imaging method, named as the sonodynamic diagnosis (SDD), can be established by the sonodynamic action with the assistance of SCL probe. Therefore, this technique could have potential applications in the clinical diagnosis of cancer diseases at an early stage, and can be used to assess the treatment efficacy during the sonodynamic therapy of cancers.
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Xiaoyuan Li and Da Xing "Sonodynamic diagnosis of cancer with sonosensitization of ATX-70 mediated by chemiluminescence probe", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005);

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