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9 February 2005 AFM imaging of proteus species
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With the fast development of scanning probe microscope, especially atomic force microscope (AFM), an imaging tool for life sciences researches is provided. Biological species imaging is one of the fundamental studies in life sciences, so it becomes one of the most important applications of AFM imaging. In this paper, AFM images of proteus species separately by contact-mode and intermittent-contact MacMode are obtained and investigated, and also are compared with its image of scanning electron microscope (SEM). Note that flagella are presented in SEM image while no evidence of flagella is observed when proteus species were imaged in AFM. This difference may be having something to do with sample preparation. The other possibility of this difference is that the proteus species which imaged was immature. Moreover, the images by MacMode in liquid medium show the outer surface of proteus species is smooth while the images by contact-mode in air show folding of the surface. The latter has clearly greater resolution than the former.
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Shi Pan, Wei Sun, Lingfeng Song, Yi Zhang, and Anhong Ning "AFM imaging of proteus species", Proc. SPIE 5635, Nanophotonics, Nanostructure, and Nanometrology, (9 February 2005);

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