Influence of the bandwidth of an acquisition system on the trap stiffness measurement

Zan Gong; Hongtao Chen; Shenghua Xu; Yinmei Li; Liren Lou

doi:10.1117/12.569877

9 February 2005 Influence of the bandwidth of an acquisition system on the trap stiffness measurement

Zan Gong, Hongtao Chen, Shenghua Xu, Yinmei Li, Liren Lou

Proceedings Volume 5635, Nanophotonics, Nanostructure, and Nanometrology; (2005) https://doi.org/10.1117/12.569877
Event: Photonics Asia, 2004, Beijing, China

Abstract

The high precision calibration of optical trap stiffness is the foundation of the weak force measurement in optical tweezers system. And the accuracy of the trap stiffness measurement is limited by the bandwidth of the acquisition system. In this article, such an influence is analyzed and discussed. First, the power spectrum of the thermal motion of a trapped bead is analyzed and the true trap stiffness is compared with the stiffness measured by a limited bandwidth acquisition system. Then the stiffness measuring process is simulated using Monte Carlo method when thermal motion analysis method is used to measure the trap stiffness. It is demonstrated that the influence of the bandwidth is related to the trap stiffness and bead diameter. And that the measured trap stiffness is greater than the true value is also demonstrated when the bandwidth of the acquisition system is not sufficient.

Citation Download Citation

Zan Gong, Hongtao Chen, Shenghua Xu, Yinmei Li, and Liren Lou "Influence of the bandwidth of an acquisition system on the trap stiffness measurement", Proc. SPIE 5635, Nanophotonics, Nanostructure, and Nanometrology, (9 February 2005); https://doi.org/10.1117/12.569877

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available