The analysis of a confocal fluorescent microscope with laser scanning microarray system

Wu Yuan; Jun-Shan Ma; Dongxiang Fu; Linlin Hou; Jiabi Chen

doi:10.1117/12.570404

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7 February 2005 The analysis of a confocal fluorescent microscope with laser scanning microarray system

Wu Yuan; Jun-Shan Ma; Dongxiang Fu; Linlin Hou; Jiabi Chen

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Proceedings Volume 5636, Holography, Diffractive Optics, and Applications II; (2005) https://doi.org/10.1117/12.570404
Event: Photonics Asia, 2004, Beijing, China

Abstract

This paper concerns on the Optical Transfer Function (OTF) of confocal fluorescent microscope in laser scanning microarray system. The cut-off of confocal microscopy, the lateral and the lognitudinal resolution, and the relationship of pinhole and resolution were discussed in a novel method. At the same time, all of these parameters of confocal microscope in microarray system are compared with other conventional and coherent microscope. The results obtained in this paper are significant for selecting the appropriate parameters and evaluating the properties of a confocal scanning fluorescent microscope for microarray reader system in order to improve the performance.

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Wu Yuan, Jun-Shan Ma, Dongxiang Fu, Linlin Hou, and Jiabi Chen "The analysis of a confocal fluorescent microscope with laser scanning microarray system", Proc. SPIE 5636, Holography, Diffractive Optics, and Applications II, (7 February 2005); https://doi.org/10.1117/12.570404