The design and fabrication of an inverted IR optical trap

Tianchun Zhu; Xiuzhou Feng; Jianxing Fang

doi:10.1117/12.574875

10 February 2005 The design and fabrication of an inverted IR optical trap

Tianchun Zhu, Xiuzhou Feng, Jianxing Fang

Proceedings Volume 5638, Optical Design and Testing II; (2005) https://doi.org/10.1117/12.574875
Event: Photonics Asia, 2004, Beijing, China

Abstract

Optical tweezers offer the unique ability to manipulate particles dispersed in a liquid medium without any mechanical contact. It can trap, move and position a wide variety of living cells and sub-cellular particles. The nature of the technique has led to its predominant use in the fields of medicine and microbiology. On the other hand, different biomedical experiments require the traps with different structures and characteristics. Commercial optical tweezers are very expensive and they can’t meet the demands of some special experiments. In this paper, the authors describe a detailed recipe for fabrication of an inverted optical trap. The system uses a single mode laser with the wavelength of 1064 nm so as not to damage the living organisms. The system has a platform whose temperature is tunable at a range of 20-40°C and can be stabilized by a controller. The system is also has a video device. The significant advantage of the system is low cost and easy to be operated. It especially fits the labs that are short of fund but interested in the application of optical trap in research of living cells. By means of the system, the authors do the experiments on control over the neuronal growth successfully.

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Tianchun Zhu, Xiuzhou Feng, and Jianxing Fang "The design and fabrication of an inverted IR optical trap", Proc. SPIE 5638, Optical Design and Testing II, (10 February 2005); https://doi.org/10.1117/12.574875

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KEYWORDS

Optical tweezers

Particles

Microscopes

Infrared lasers

Objectives

Charge-coupled devices

Optical filters

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