Infrared imaging of normal and diseased cervical tissue sections

Bayden R. Wood; Keith R. Bambery; Lisa M. Miller; Michael Quinn M.D.; Luis Chiriboga; Max Diem; Don McNaughton

doi:10.1117/12.582294

16 February 2005 Infrared imaging of normal and diseased cervical tissue sections

Bayden R. Wood, Keith R. Bambery, Lisa M. Miller, Michael Quinn M.D., Luis Chiriboga, Max Diem, Don McNaughton

Proceedings Volume 5651, Biomedical Applications of Micro- and Nanoengineering II; (2005) https://doi.org/10.1117/12.582294
Event: Smart Materials, Nano-, and Micro-Smart Systems, 2004, Sydney, Australia

Abstract

Synchrotron FTIR maps, focal plane array and linear array images recorded of 4 μm cervical biopsy sections from the surface epithelium and glandular endometrium are compared in terms of spatial resolution and applicability to the clinical environment. Synchrotron FTIR maps using a 10 μm aperture appear to provide a better spatial resolution capable of discerning single nuclei in the tissue matrix. Unsupervised hierarchical cluster analysis performed on the synchrotron, focal plane array and linear array data in the 1700-1400 cm^-1 region show very similar clusters and mean-extracted spectra, demonstrating the robustness of FTIR microscopy and UHCA in the analysis of tissue sections. Maps recorded with the focal plane array using a conventional globar source take one-fortieth of the time but the spatial resolution precludes true single cell analysis in the tissue matrix. The high spatial resolution achieved with the synchrotron shows potential as a gold standard for FTIR diagnosis of cervical samples.

Citation Download Citation

Bayden R. Wood, Keith R. Bambery, Lisa M. Miller, Michael Quinn M.D., Luis Chiriboga, Max Diem, and Don McNaughton "Infrared imaging of normal and diseased cervical tissue sections", Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); https://doi.org/10.1117/12.582294

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