8 October 2004 Novel approach to process protein crystals using deep-UV laser
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Proceedings Volume 5662, Fifth International Symposium on Laser Precision Microfabrication; (2004) https://doi.org/10.1117/12.596331
Event: Fifth International Symposium on Laser Precision Microfabrication, 2004, Nara, Japan
Abstract
We propose new application of a pulsed deep-UV laser to processing biological macromolecule crystals. Single crystals of model protein, hen egg white lysozyme, were processed by deep-UV laser irradiation at 193 nm and 266 nm. Desired laser ablation was achieved using the 193-nm light source. A crystallinity of the processed crystals was evaluated by X-ray diffraction pattern recording. The crystal diffracted beyond a 1.9 Å resolution, which was the same as that of the as-grown crystal obtained under identical growth conditions. In addition, we observed that a diffraction pattern from a cracked protein crystal was considerably improved by eliminating the damaged section with the 193-nm laser ablation. A processing tool for protein crystals currently employed, such as a micro needle or knife, suffers from low reproducibility and poor accuracy. The technique using deep-UV laser pulses will be a powerful tool for processing very fragile protein crystals.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hiroshi Kitano, Hiroshi Kitano, Atsushi Sato, Atsushi Sato, Atsutoshi Murakami, Atsutoshi Murakami, Hiroaki Adachi, Hiroaki Adachi, Hiroyoshi Matsumura, Hiroyoshi Matsumura, Kazufumi Takano, Kazufumi Takano, Tsuyoshi Inoue, Tsuyoshi Inoue, Yusuke Mori, Yusuke Mori, Masaaki Doi, Masaaki Doi, Takatomo Sasaki, Takatomo Sasaki, } "Novel approach to process protein crystals using deep-UV laser", Proc. SPIE 5662, Fifth International Symposium on Laser Precision Microfabrication, (8 October 2004); doi: 10.1117/12.596331; https://doi.org/10.1117/12.596331
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