In vivo multiphoton tomography of skin as a tool to study the effects of topically applied probes and UV exposure

Iris Riemann; Alexander Ehlers; Annette Reif; Jens Kobow; Karsten Konig

doi:10.1117/12.590817

25 April 2005 In vivo multiphoton tomography of skin as a tool to study the effects of topically applied probes and UV exposure

Iris Riemann, Alexander Ehlers, Annette Reif, Jens Kobow, Karsten Konig

Author Affiliations +

Proceedings Volume 5686, Photonic Therapeutics and Diagnostics; (2005) https://doi.org/10.1117/12.590817
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

High resolution optical tomography studies on topically applied probes and UVA exposed skin were performed with the novel imaging system DermaInspect. 3D images with submicron resolution based on the two-photon excitation of the probes and the autofluorescence of epidermal cells and structures with femtosecond laser pulses in the near infrared (NIR) spectral range between 750 nm and 850 nm were obtained. The distribution of natural endogenous fluorophores, mainly keratin and NAD(P)H, as well as of the exogenous probe molecules was detected in situ. The high resolution imaging system DermaInspect offers an unique chance of in situ, non-invasive drug screening in skin on a molecular level and to study the effect of UV exposure.

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Iris Riemann, Alexander Ehlers, Annette Reif, Jens Kobow, and Karsten Konig "In vivo multiphoton tomography of skin as a tool to study the effects of topically applied probes and UV exposure", Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); https://doi.org/10.1117/12.590817

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