25 April 2005 Preliminary observations on differences in the Raman spectra of cancerous and noncancerous cells and connective tissue of human skin
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Abstract
A less invasive method of reliably detecting skin cancers is required. Raman spectroscopy is just one of several spectroscopic methods that look promising, but are not yet sufficiently reliable. More information is needed on how and why the Raman spectra of cancerous skin tissue is different from its normal counterpart. We have used confocal micro-Raman spectroscopy with a spatial resolution of about a micron to obtain spectra of unstained thin sections of human skin. We found that there were clear differences in the Raman spectra between cancerous and non-cancerous tissue both in cells and in the connective tissue. The DNA contribution to the spectra was generally stronger in malignant cells than normal ones. In regions of the dermis far away from the tumor one obtains the usual collagen spectra of normal skin, but adjacent to the tumor the spectra no longer appeared to be those of native collagen.
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Michael A. Short, Harvey Lui, David I. McLean, Haishan Zeng, Abdulmajeed Alajlan, Michael X. Chen, "Preliminary observations on differences in the Raman spectra of cancerous and noncancerous cells and connective tissue of human skin", Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.591076; https://doi.org/10.1117/12.591076
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