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18 April 2005 Estimation of melanin content in iris of human eye
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Abstract
Based on experimental data, obtained in vitro from reflectance measurements and in vivo from digital analysis of color images of human irises, melanin content in human and bovine eye irises has been estimated. Reflectance measurements have been performed using commercially available optical multichannel spectrometer LESA-5 (BioSpec, Russia). For registration of color images digital camera Olympus C-5060 has been used. Analysis of the reflectance spectra has been performed by the method used for determination of melanin content in skin. For digital analysis of iris color images, decomposition of the images in RGB-color-coordinate system has been performed. The images have been obtained both from irises of health volunteers as from irises of patients with glaucoma. Original computer program based on Mathcad software has been developed for the analysis. The results obtained from spectral and color measurements have a good agreement each to other. In eye irises of patients with glaucoma, smaller melanin content has been obtained, and the result has been useful for development of novel and optimization of already existing methods of glaucoma diagnostics.
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Alexey N. Bashkatov, Ekaterina V. Koblova, Valery V. Bakutkin, Elina A. Genina, Eugeny P. Savchenko, and Valery V. Tuchin "Estimation of melanin content in iris of human eye", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); https://doi.org/10.1117/12.593651
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