18 April 2005 Full-field OCT: applications in ophthalmology
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Abstract
We present images of ocular tissues obtained using ultrahigh resolution full-field OCT. The experimental setup is based on the Linnik interferometer, illuminated by a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a high-resolution CCD camera. A spatial resolution of 0.7 μm × 0.9 μm (axial × transverse) is achieved thanks to the short source coherence length and the use of high numerical aperture microscope objectives. A detection sensitivity of 90 dB is obtained by means of image averaging and pixel binning. Whole unfixed eyes and unstained tissue samples (cornea, lens, retina, choroid and sclera) of ex vivo rat, mouse, rabbit and porcine ocular tissues were examined. The unprecedented resolution of our instrument allows cellular-level resolution in the cornea and retina, and visualization of individual fibers in the lens. Transcorneal lens imaging was possible in all animals, and in albino animals, transscleral retinal imaging was achieved. We also introduce our rapid acquisition full-field optical coherence tomography system designed to accommodate in vivo ophthalmologic imaging. The variations on the original system technology include the introduction of a xenon arc lamp as source, and rapid image acquisition performed by a high-speed CMOS camera, reducing acquisition time to 5 ms per frame.
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Katharine Grieve, Katharine Grieve, Arnaud Dubois, Arnaud Dubois, Michel Paques, Michel Paques, Jean-François Le Gargasson, Jean-François Le Gargasson, Albert Claude Boccara, Albert Claude Boccara, } "Full-field OCT: applications in ophthalmology", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); doi: 10.1117/12.588649; https://doi.org/10.1117/12.588649
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