Light scattering reports early mitochondrial responses to photodynamic therapy

Jeremy D. Wilson; Thomas H. Foster

doi:10.1117/12.588729

8 April 2005 Light scattering reports early mitochondrial responses to photodynamic therapy (Invited paper)

Jeremy D. Wilson, Thomas H. Foster

Proceedings Volume 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV; (2005) https://doi.org/10.1117/12.588729
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Angularly-resolved light scattering is an established method of particle sizing. Scattering from intact cells provides information about the size distributions of intracellular scatterers. Mitochondria are important light scatterers, especially at forward angles. Nuclei play an important part in scattering light at extreme forward angles and in backscattering geometries. Because changes in mitochondrial morphology are among the early responses to photodynamic therapy (PDT) using mitochondrial-localizing sensitizers and because these changes may be important in determining the fate of the cell, it is interesting to consider light scattering as a means of assessing the response of cells and tissue to PDT. Simple transmission measurements in an absorption spectrophotometer report a rapid reduction in scattering in cells subjected to aminolevulinic acid (ALA)-PDT. ALA-PDT with a fluence of 5 J cm^-2 induces a change in the angularly-resolved light scattering from EMT6 cells in suspension within approximately 45 minutes of irradiation. At earlier times following this fluence, the scattering differs only slightly from that observed with control cells. Analysis of the post-treatment scattering data at forward angles is consistent with mitochondrial swelling. Qualitatively similar changes in scattering are observed immediately after a fluence of 10 J cm^-2 in cells sensitized with Pc 4.

Citation Download Citation

Jeremy D. Wilson and Thomas H. Foster "Light scattering reports early mitochondrial responses to photodynamic therapy (Invited paper)", Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); https://doi.org/10.1117/12.588729

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